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The theory in the cTnT measurement is undoubtedly an ELISA 1-step sandwich assay using streptavidin know-how and electrochemiluminescence detection.[54] In the 1st incubation step (immunological reaction), the cTnT through the sample reacts having a biotinylated mouse monoclonal anti-cTnT antibody in addition to a monoclonal cTnT-certain antibody labeled having a ruthenium complicated to kind a sandwich complex.

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This IgG ought to neutralize the greater regularly encountered HAMA.[ninety] Measures the laboratory normally takes To guage this likelihood involve applying a bigger dilution in the sample that has a reagent containing the non-immune mouse IgG or undertaking the Evaluation over a in another way configured assay, preferably just one employing diverse species of reagent antibodies.[91]

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